human nectin2  (VectorBuilder GmbH)

Journal: Cell Discovery

Article Title: Stromal architecture and fibroblast subpopulations with opposing effects on outcomes in hepatocellular carcinoma

doi: 10.1038/s41421-024-00747-z

Figure Lengend Snippet: a UMAPs showing tissue origin of fibroblasts from human perturbed-state fibroblasts atlas (left) and their expression of CAF-FAP (middle) and CAF-C7 (right) signature. b Schematic diagram of integrating scRNA-seq data of human fibroblasts collected from different cancer types and locations. c Estimated CAF-FAP (upper) and CAF-C7 (lower) abundance in pan-cancer fibroblasts, showing the expression of subcluster signature among cancer types. d Pearson correlation between CAF-FAP and CD8_PDCD1 abundance and between FAP and PDCD1 gene expression of TCGA data across 32 solid tumor types. Cell abundance was quantified by mean expression of the top 50 DEGs. Pearson correlation. e Kaplan-Meier plot showing the overall survival across 6 cancer types from TCGA based on the mean expression of TIGIT and NECTIN2 . Log-rank test. f Characterization of the inflammatory hubs in published ST data of human breast, ovarian, and endometrial cancer, showing paired histology, spatial distribution, and correlation of CAF-FAP and CD8_PDCD1, pathway activity, marker gene expression, and ligand‒receptor pairs. Pearson correlation. g Schematic diagram of the two major stromal archetypes (FR + and FR ‒ ) in HCC, displaying broad differences between FR + and FR ‒ tumors regarding fibroblast heterogeneity, multicellular interactome, and ECM properties.

Article Snippet: NECTIN2 -overexpressing ( NECTIN2 -OE) and control LX-2 cells were cultured in confocal dishes and stained with DIO (MCE) 24 h post-transfection.

Techniques: Expressing, Gene Expression, Activity Assay, Marker

Journal: Cancer Cell

Article Title: Integrative analysis of neuroblastoma by single-cell RNA sequencing identifies the NECTIN2-TIGIT axis as a target for immunotherapy

doi: 10.1016/j.ccell.2023.12.008

Figure Lengend Snippet:

Article Snippet: Recomb Anti-NECTIN2 PE-Vio615 , Miltenyi , Cat# 130-122-784; RRID: AB_2819430.

Techniques: Recombinant, Modification, Staining, RNA Sequencing, Transduction, Software

Journal: Cell reports

Article Title: PTK7 is a positive allosteric modulator of GPR133 signaling in glioblastoma

doi: 10.1016/j.celrep.2023.112679

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Ligand candidate cDNAs were obtained from the following commercially available plasmids: PTK7 (Addgene, Cat#65250), PCDHGC3 (SinoBiological, Cat#HG22064-UT), TFRC (Addgene, Cat#69610), NECTIN2 (SinoBiological, Cat#HG10005-G), CADM4 (SinoBiological, Cat#HG16033-G), MCAM (SinoBiological, Cat#HG10115-M), PLXDC2 (SinoBiological, Cat#HG23536-U).

Techniques: Recombinant, Magnetic Beads, Blocking Assay, Enzyme-linked Immunosorbent Assay, Mass Spectrometry, Derivative Assay, Plasmid Preparation, Software